Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Confocal fluorescence polarization microscopy in turbid media: effects of scattering-induced depolarization

Not Accessible

Your library or personal account may give you access

Abstract

We present an experimental and theoretical study of confocal fluorescence polarization microscopy in turbid media. We have performed an experimental study using a fluorophore-embedded polymer rod immersed in aqueous suspensions of 0.1 and 0.5μm diameter polystyrene microspheres. A Monte Carlo approach to simulate confocal fluorescence polarization imaging in scattering media is also presented. It incorporates a detailed model of polarized fluorescence generation that includes sampling of elliptical polarization, excited-state molecular rotational Brownian motion, and dipole fluorescence emission. Using both approaches, we determine the effects of the number of scattering events, target depth, photon scattering statistics, objective numerical aperture, and pinhole size on confocal anisotropy imaging. From this detailed analysis and comparison of experiment with simulation, we determine that fluorescence polarization is maintained to depths at which meaningful intensity images can be acquired.

© 2006 Optical Society of America

Full Article  |  PDF Article
More Like This
Confocal microscopy in turbid media

J.M. Schmitt, A. Knüttel, and M. Yadlowsky
J. Opt. Soc. Am. A 11(8) 2226-2235 (1994)

Measuring the scattering coefficient of turbid media from two-photon microscopy

David Sevrain, Matthieu Dubreuil, Aymeric Leray, Christophe Odin, and Yann Le Grand
Opt. Express 21(21) 25221-25235 (2013)

Characterizing the depolarization of circularly polarized light in turbid scattering media

Callum M. Macdonald
J. Opt. Soc. Am. A 35(12) 2104-2110 (2018)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (9)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Tables (1)

You do not have subscription access to this journal. Article tables are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (16)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved