Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Three-dimensional optical-transfer-function analysis for a laser-scan fluorescence microscope with an extended detector

Not Accessible

Your library or personal account may give you access

Abstract

Three-dimensional imaging characteristics of a laser-scan fluorescence microscope with various sizes of detector are quantitatively analyzed through the studies and calculations of the three-dimensional optical transfer function of the microscope. It is found that a detector of an Airy-disk size formed by the objective lens in the detector plane provides both reasonable energy-collection efficiency and three-dimensionally resolved imaging capability. It is also found that a slit detector provides longitudinal resolution of the sample structure similarly to a confocal microscope, while the lateral resolution is approximately the same as that of a conventional microscope.

© 1991 Optical Society of America

Full Article  |  PDF Article
More Like This
Calculation of three-dimensional optical transfer function for a confocal scanning fluorescent microscope

Shigeharu Kimura and Chusuke Munakata
J. Opt. Soc. Am. A 6(7) 1015-1019 (1989)

Three-dimensional coherent transfer functions in fiber-optical confocal scanning microscopes

Min Gu, X. Gan, and C. J. R. Sheppard
J. Opt. Soc. Am. A 8(7) 1019-1025 (1991)

Three-dimensional transfer-function analysis of the tomographic capability of a confocal fluorescence microscope

Osamu Nakamura and Satoshi Kawata
J. Opt. Soc. Am. A 7(3) 522-526 (1990)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (5)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Equations (9)

You do not have subscription access to this journal. Equations are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved